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Materials and methods Cell culture Human immortalized keratinocytes (HaCaT) were procured from Bo Gu (Shanghai, China), and cultured in 1640 medium supplemented with 10% FBS and 100 g/mL penicillin and streptomycin at 37 C in air supplemented with 5% CO 2

Excessive Alcohol Consumption: High alcohol intake burdens the liver, where glutathione is concentrated

As can be seen from Volcano Figure 6 , the FI group up-regulated 6 differential metabolites and down-regulated 12 differential metabolites relative to the NC group

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